Journal: British Journal of Cancer

Article Title: PPAR γ agonists regulate the expression of stemness and differentiation genes in brain tumour stem cells

doi: 10.1038/bjc.2012.161

Figure Lengend Snippet: Alteration of differentiation and stemness genes in BTSCs by PPAR γ agonists. T98G- and DB29-BTSCs were cultured in NMB in the presence of 10 μ ℳ ciglitazone. Total RNA was extracted after 24 h, and the gene expression profile determined by qRT−PCR using TaqMan low-density optical 384-well human stem cell gene panel. Gene expression in DB29- and T98G-BTSCs was normalised to 18S and GAPDH, respectively. The heat map ( A ) was constructed using DataAssist software. Box plots ( B ), scatter plots ( C ), and Venn diagrams ( D ) were generated using GraphPad Prism 5.0 software. The differentiation genes and stemness genes are shown in red and green, respectively. Lines in the box plots represent the median and the numbers represent the mean. ( E ) Total cell lysates were prepared from T98G and DB29 glioma, and BTSCs treated with 10 μ ℳ ciglitazone or 15d-PGJ2 for 24 h, and the expression of GFAP, NG2, β −III Tubulin, CD133, and β -actin was determined by western blot. Numbers represent the relative quantities of protein calculated using β -actin as internal control. The figure is a representative of two independent experiments.

Article Snippet: Box plot, scatter plot, and Venn diagrams were generated using GraphPad Prism 5.0 software (GraphPad, La Jolla, CA, USA).

Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Construct, Software, Generated, Western Blot